rgc axon projections  (Stryker)

Journal:

Article Title: The immune protein CD3? is required for normal development of neural circuits in the retina

doi: 10.1016/j.neuron.2010.01.035

Figure Lengend Snippet: (A) Representative fluorescent images of ipsilateral (upper) and contralateral (middle) RGC axon projections to the dLGN of WT and CD3ζ-/- mice at P8 and P16. The lower row shows an overlay of the two projections, with ipsilateral in red and contralateral in green. (B) Summary quantification of RGC axon projections of WT, CD3ζ+/- and CD3ζ-/- mice at P8 and P16, show the fraction of contralateral, ipsilateral and overlap projections in the dLGN and the Segregation Index (mean variance). Axons are similarly segregated at P8, but axons, especially for ipsilateral projections, occupy larger area of dLGN in CD3ζ+/- than in WT mice at P16 and overlapped with projections from the contralateral eye. See also Fig S3.

Article Snippet: Blocking spontaneous or light evoked retinal activity retards the emergence of RGC dendritic stratification in the retina ( Bansal et al ., 2000 ; Bodnarenko et al ., 1993 ; Tian and Copenhagen, 2003 ; Wong et al ., 2000 ; Wong and Ghosh, 2002 ; Xu and Tian, 2007 ), eye-specific segregation and retinotopic map of RGC axonal projections to the dLGN ( Rossi et al ., 2001 ; Akerman et al ., 2002 ; Chapman, 2000 ; Grubb et al ., 2004 ; Huberman et al ., 2003 ; Muir-Robinson et al ., 2002 ; Penn et al ., 1998 ; Shatz and Stryker, 1988 ; Torborg et al ., 2005 ; Grubb et al ., 2004 ) and superior colliculus ( Rossi et al ., 2001 ; Chandrasekaran et al ., 2005 ; McLaughlin et al ., 2003 ; Mrsic-Flogel et al ., 2005 ), the development of precise dLGN projections to visual cortex ( Stryker and Harris, 1986 ; Cang et al ., 2005 ; Huberman et al ., 2006 ), and the dendritic/axonal growth of neurons in the visual system ( Majewska and Sur, 2003 ; Oray et al ., 2004 ; Parnavelas et al ., 1973 ; Riccio and Matthews, 1985 ; Wallace and Bear, 2004 ).

Techniques:

Journal:

Article Title: The immune protein CD3? is required for normal development of neural circuits in the retina

doi: 10.1016/j.neuron.2010.01.035

Figure Lengend Snippet: Blockade of glutamatergic synaptic transmission in WT retina disrupts eye specific segregation of RGC axons to the dLGN, mimicking the effects of genetic deletion of CD3ζ. (A) Representative fluorescent images of ipsilateral (red) and contralateral (green) RGC axon projections to the dLGN of P14 WT mice that received daily binocular injections of either saline (top) or NBQX+AP5 (bottom) from P7-P12. Quantification of the fraction of contralateral (B), ipsilateral (C) and overlap (D) projections in the dLGN and the Segregation Index (E) in WT mice at P14 with or without NBQX+AP5 treatment. Intraocular treatment with glutamate receptor antagonists disrupted e eye-specific segregation compared with saline treated controls. (F-H) Blocking glutamatergic neurotransmission in WT retina fully recapitulates the reduced RGC dendritic pruning observed in CD3ζ-/- mice. (F) The average density of dendritic protrusions is higher in A1 and A2 RGCs in P12 WT mice that have had chronic intraocular application of GluR antagonists (NBQX and AP5) from P7-P11. Application of the GluR antagonists to CD3ζ-/- mice has no additional effect. (G) Cumulative distribution of A1 RGC dendritic kinetics from WT retinas in control solution, WT retinas in the presence of 10 μM NBQX + 100 μM AP5, and CD3ζ-/- retinas in control solution. Bath application of NBQX + AP5 reduced the dynamics of filopodia extension and retraction to the same level as that observed in CD3ζ-/- retinas. (H) Blockade of glutamate neurotransmission increased the average lifetime of RGC filopodia in WT mice, mimicking the effects induced by genetic deletion of CD3ζ. Please note that the data shown in this figure was collected using a regular epifluorescent microscope while the data shown in figure 7 was collected using a laser confocal microscope. Therefore, the actual values of the result, especially the ipsilateral staining, are different in these two figures.

Article Snippet: Blocking spontaneous or light evoked retinal activity retards the emergence of RGC dendritic stratification in the retina ( Bansal et al ., 2000 ; Bodnarenko et al ., 1993 ; Tian and Copenhagen, 2003 ; Wong et al ., 2000 ; Wong and Ghosh, 2002 ; Xu and Tian, 2007 ), eye-specific segregation and retinotopic map of RGC axonal projections to the dLGN ( Rossi et al ., 2001 ; Akerman et al ., 2002 ; Chapman, 2000 ; Grubb et al ., 2004 ; Huberman et al ., 2003 ; Muir-Robinson et al ., 2002 ; Penn et al ., 1998 ; Shatz and Stryker, 1988 ; Torborg et al ., 2005 ; Grubb et al ., 2004 ) and superior colliculus ( Rossi et al ., 2001 ; Chandrasekaran et al ., 2005 ; McLaughlin et al ., 2003 ; Mrsic-Flogel et al ., 2005 ), the development of precise dLGN projections to visual cortex ( Stryker and Harris, 1986 ; Cang et al ., 2005 ; Huberman et al ., 2006 ), and the dendritic/axonal growth of neurons in the visual system ( Majewska and Sur, 2003 ; Oray et al ., 2004 ; Parnavelas et al ., 1973 ; Riccio and Matthews, 1985 ; Wallace and Bear, 2004 ).

Techniques: Transmission Assay, Saline, Blocking Assay, Control, Microscopy, Staining